Null mutants of Saccharomyces cerevisiae Cu,Zn superoxide dismutase: characterization and spontaneous mutation rates.
نویسندگان
چکیده
Deletion-replacement mutations of the Saccharomyces cerevisiae Cu,Zn superoxide dismutase gene were constructed. They were exquisitely sensitive to redox cycling drugs and showed slight sensitivity to other agents. The aerobic spontaneous mutation rate was three- to fourfold higher in sod1 delta 1 mutants, while the anaerobic rate was similar to that of the wild type.
منابع مشابه
Antioxidant functions required for insusceptibility of Saccharomyces cerevisiae to tetracycline antibiotics.
Cu,Zn superoxide dismutase (Sod1) is required for insusceptibility of Saccharomyces cerevisiae to oxytetracycline (OTC). Here we report that Sod1 is also required for insusceptibility to doxycycline (DOX). Furthermore, among a range of antioxidant and redox balance mutants, mac1 and ctr1 deletion strains exhibited marked sensitization to OTC and DOX. Certain mutants exhibited a slight sensitivi...
متن کاملThe involvement of GSH in the activation of human Sod1 linked to FALS in chronologically aged yeast cells.
Mutations in Cu, Zn-superoxide dismutase (Sod1) have been associated with familial amyotrophic lateral sclerosis, an age-related disease. Because several studies suggest that oxidative stress plays a central role in neurodegeneration, we aimed to investigate the role of the antioxidant glutathione (GSH) in the activation of human A4V Sod1 during chronological aging. Transformation of wild-type ...
متن کاملSuperoxide dismutase (sod-1) null mutants of Neurospora crassa: oxidative stress sensitivity, spontaneous mutation rate and response to mutagens.
Enzymatic superoxide-dismutase activity is believed to be important in defense against the toxic effects of superoxide. Although superoxide dismutases are among the best studied proteins, numerous questions remain concerning the specific biological roles of the various superoxide-dismutase types. In part, this is because the proposed damaging effects of superoxide are manifold, ranging from ina...
متن کاملMechanisms for activating Cu- and Zn-containing superoxide dismutase in the absence of the CCS Cu chaperone.
The Cu- and Zn-containing superoxide dismutase 1 (SOD1) largely obtains Cu in vivo by means of the action of the Cu chaperone CCS. Yet, in the case of mammalian SOD1, a secondary pathway of activation is apparent. Specifically, when human SOD1 is expressed in either yeast or mammalian cells that are null for CCS, the SOD1 enzyme retains a certain degree of activity. This CCS-independent activit...
متن کاملESSENTIAL ARGINYL RESIDUES IN Cu,Zn SUPEROXIDE DISMUTASE FROM SACCHAROMYCES CEREVISIAE
Cu,Zn superoxide dismutase from baker's yeast, Saccharomyces cerevisiae, is inactivated by either butanedione in borate buffer or phenylglyoxal. Inactivation by butanedione takes place only in the presence of borate, and butanedione-inactivation is reversed on removal of borate. Initial studies with phenylglyoxal suggested that inactivation is due to the modification of 0.5 arginyl residues per...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of bacteriology
دوره 173 18 شماره
صفحات -
تاریخ انتشار 1991